Associate Scientist, Next Generation Sequencing

Company: Initial Therapeutics, Inc.
Location: Boston
Posted on: April 2, 2025

Job Description:

By clicking the "Apply" button, I understand that my employment application process with Takeda will commence and that the information I provide in my application will be processed in line with Takeda's and . I further attest that all information I submit in my employment application is true to the best of my knowledge.Job DescriptionAbout the role:At Takeda, we are a forward-looking, world-class R&D organization that unlocks innovation and delivers transformative therapies to patients. By focusing R&D efforts on four therapeutic areas and other targeted investments, we push the boundaries of what is possible in order to bring life-changing therapies to patients worldwide.As an Associate Scientist, Next Generation Sequencing, you will deliver on the priorities of Global Biologics research and Biologics Engineering and Antibody Discovery organization by contributing to the discovery, optimization and engineering of the antibody and CAR-T cells by deep mining repertoire of therapeutic antibodies using NGS to accelerate programs to IND.How you will contribute:

• Generation of NGS library (RNAseq, amplicon), from single cell platform (10X, Rhapsody, Parse Bioscience, PIPseq, plate-based) or bulk B cells, perform library QC, library quantification and running/operating Miseq/Nextseq.
• Use molecular biology laboratory best practices for RNA isolation, cDNA synthesis, PCR amplification, SPRI bead based clean up and fragment analysis.
• Serve as a subject matter expert for library generation and work with a broad enterprise mindset.
• Strategize best workflow and design NGS primers to utilize NGS sequencing tools.
• Ability to manage several competing high priority project simultaneously.
• Document laboratory work using electronic laboratory notebook and follow the previously established protocol.
• Lead and evaluate the emerging single cell library generation and DNA sequencing platforms.
• Optimize, improve and troubleshoot NGS related workflows to get better sequencing data.
• Work independently with efficiency, agility, speed and collaborate to thrive in a high pace, high impact team environment.Minimum Requirements/Qualifications:
  • MS with 3+ years and BS with 5+ years experience.
  • A previous experience with building NGS libraries to deeply mine the antibody repertoires sequences is required.
  • Excellent knowledge of details on the platform source of antibodies, repertoires (e.g. transgenic mice/display platforms; immune or naive repertoires) and their nuances is highly desirable.
  • A strong background in molecular biology and able to work with RNA/DNA/Plasmids or cells as the starting material.
  • Previous experience generating high quality NGS library as established by rigorous PCR condition optimization, QC process and troubleshooting.
  • Experience performing DNA fragment purification using Blue Pippin and analyze the size using Tape Station 4200. Library quantification using qubit flex and qPCR.
  • Knowledge of MiSeq/Nextseq sequencing operation and maintenance.More about us:At Takeda, we are transforming patient care through the development of novel specialty pharmaceuticals and best in class patient support programs. Takeda is a patient-focused company that will inspire and empower you to grow through life-changing work.Certified as a Global Top Employer, Takeda offers stimulating careers, encourages innovation, and strives for excellence in everything we do. We foster an inclusive, collaborative workplace, in which our teams are united by an unwavering commitment to deliver Better Health and a Brighter Future to people around the world.Takeda Compensation and Benefits SummaryWe understand compensation is an important factor as you consider the next step in your career. We are committed to equitable pay for all employees, and we strive to be more transparent with our pay practices.For Location:Boston, MAU.S. Base Salary Range:$96,600.00 - $151,800.00The estimated salary range reflects an anticipated range for this position. The actual base salary offered may depend on a variety of factors, including the qualifications of the individual applicant for the position, years of relevant experience, specific and unique skills, level of education attained, certifications or other professional licenses held, and the location in which the applicant lives and/or from which they will be performing the job. The actual base salary offered will be in accordance with state or local minimum wage requirements for the job location.U.S. based employees may be eligible for short-term and/or long-term incentives. U.S. based employees may be eligible to participate in medical, dental, vision insurance, a 401(k) plan and company match, short-term and long-term disability coverage, basic life insurance, a tuition reimbursement program, paid volunteer time off, company holidays, and well-being benefits, among others. U.S. based employees are also eligible to receive, per calendar year, up to 80 hours of sick time, and new hires are eligible to accrue up to 120 hours of paid vacation.EEO StatementTakeda is proud in its commitment to creating a diverse workforce and providing equal employment opportunities to all employees and applicants for employment without regard to race, color, religion, sex, sexual orientation, gender identity, gender expression, parental status, national origin, age, disability, citizenship status, genetic information or characteristics, marital status, status as a Vietnam era veteran, special disabled veteran, or other protected veteran in accordance with applicable federal, state and local laws, and any other characteristic protected by law.Locations: Boston, MAWorker Type: EmployeeWorker Sub-Type: RegularTime Type: Full timeJob Exempt: YesIt is unlawful in Massachusetts to require or administer a lie detector test as a condition of employment or continued employment. An employer who violates this law shall be subject to criminal penalties and civil liability.
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Keywords: Initial Therapeutics, Inc., Boston , Associate Scientist, Next Generation Sequencing, Other , Boston, Massachusetts