Scientist II, Cell Line Development

Company: Initial Therapeutics, Inc.
Location: Boston
Posted on: February 11, 2025

Job Description:

A career at Resilience is more than just a job - it's an opportunity to change the future.
Resilience is a technology-focused biomanufacturing company that's changing the way medicine is made. We're building a sustainable network of high-tech, end-to-end manufacturing solutions to better withstand disruptive events, serve scientific discovery, and reach those in need.
For more information, please visit
Position Summary & Role
The Scientist II Cell Line Development is responsible for mammalian cell line culture and cell line development, construct development, cell line characterization and stability testing, single cell cloning, as well as optimization of assays for the Biologics, Vaccines and Gene Therapy modalities. The Scientist II should also possess knowledge and experience with viral cell line transfection and engineering, including AAV or LVV stable gene incorporation, scientific principles and concepts in fields of cell biology, protein expression, molecular biology, stable and transient transfections, clone screening, small scale bioreactors, and DNA PCR. The Scientist II will be responsible for executing experimental approaches necessary to implement corporate strategy. Experience with microbial expression is desirable.
Job Responsibilities

• 
  
• Responsible for execution of state-of-the-art technology to develop cell lines and clones.
  
• Determines required experiments for upstream processes including stable and transient transfections, selections, clone isolation, screening, scale-up, and fed-batches in shake flask.
  
• Responsible for developing and performing small-scale bioreactor runs for cell testing and production.
  
• Skilled in molecular biology including recombinant cloning and preparation of expression vectors, RT-PCR, and other standard cell biology techniques.
  
• Perform antibody and other protein quantitation and assessment using Octet, SDS-PAGE, western blots and other techniques.
  
• Assist in data analysis related to upstream processes and assays and experimental outcomes.
  
• Contribute to cell culture development efforts.
  
• Assist in research, product, and process development for current and novel work.
  
• Participate in maintaining capabilities, databases, laboratories, equipment, and supplies in the areas of protein engineering, cell biology, and cell line development.
  
• Write and review R&D protocols, SOPs, reports, and other product development and R&D documents.
  
• Support Manufacturing and other departments as needed.
  
• All other tasks as needed or assigned by supervisor.
  
  Minimum Qualifications
  
  • 
    
  • Background in mammalian cell culture including CHO suspension cultures and in mammalian cell line development.
    
  • Strong writing, analysis, and communication skills, both oral and written.
    
  • Must have excellent organizational planning skills with attention to details.
    
    Preferred Experience, Education & Qualifications
    
    • 
      
    • The ideal candidate will have a PhD in molecular biology, biochemistry, chemistry, or related field with suitable experience reflecting the skillset required in the position. An MS or BA/BS will also be considered.
      
    • Experience in process development and/or manufacturing.
      
    • Ability to connect at all levels of the organization and work well in teams.
      
    • Must be proficient in managing multiple tasks and prioritizing workload with effective independent follow-up with timely results.
      
    • Able to work in a fast-paced, results-oriented environment.
      
      Resilience is an Equal Employment Opportunity Employer. Qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender perception or identity, national origin, age, marital status, protected veteran status, disability status, physical or mental disability, genetic information, or characteristic, or other non-job-related characteristics or other prohibited grounds specified in applicable federal, state, and local laws. Requests for reasonable accommodation can be made at any stage of the recruitment process.
      Resilience offers employees a robust total rewards program including equity, an annual cash bonus program, a 401(k) plan with a generous company match and our benefits package which is thoughtfully designed to support our employees with great healthcare (including medical, dental and vision), family building benefits, life and disability insurance, flexible time off, paid holidays, other paid leaves of absence, tuition reimbursement and support for caregiving needs. Our target base pay hiring range for this position is $100,000.00 - $145,000.00 per year. Actual base pay is dependent upon a number of factors, including but not limited to, the candidate's geographical location, relevant experience, qualifications, skills and knowledge. Excited about Resilience and the biomanufacturing revolution? We encourage you to apply and start a conversation with one of our recruiters.
      #J-18808-Ljbffr

Keywords: Initial Therapeutics, Inc., Boston , Scientist II, Cell Line Development, Other , Boston, Massachusetts